[Source: Antimicrobial Agents and Chemotherapy, full page: (LINK). Abstract, edited.]
Emergence of Escherichia coli ST131 producing KPC-2 carbapenemase in China
Jia Chang Cai, Rong Zhang, Yan Yan Hu, Hong Wei Zhou and Gong-Xiang Chen
Author Affiliations: 2nd Affiliated Hospital of Zhejiang University, Zhejiang University, 88 Jiefang Rd., Hangzhou 310009, The People’s Republic of China
Published ahead of print 9 December 2013, doi: 10.1128/AAC.00912-13
Twenty-two KPC-2-producing Escherichia coli were isolated from 3 hospitals in Hangzhou from 2007 to 2011. One isolate with OmpC porin deficiency exhibited high-level carbapenem resistance. Pulsed-field gel electrophoresis showed that few isolates were indistinguishable or closely related. Multilocus sequence typing indicated that ST131 was the predominant type (9 isolates, 40.9%), followed by ST648 (5 isolates), ST405 (2 isolates), ST38 (2 isolates), and 4 single STs of ST69, ST2003, ST2179, and ST744. Phylogenetic analysis indicated that 9 group B2 isolates belonged to ST131, 5 of 11 group D isolates belonged to ST648. Only one group B1 isolate and one group A isolate were identified. A representative plasmid (pE1) were partially sequenced and a 7,788-bp DNA fragment encoding Tn3 transposase, Tn3 resolvase, ISKpn8 transposase, KPC-2, and ISKpn6-like transposase was obtained. The blaKPC-2-surrounding sequence was amplified by a series of primers. The PCR results showed that 13 isolates were consistent with the genetic environment in pE1. It is the first report of rapid emergence of KPC-2-producing E. coli ST131 in China. blaKPC-2 gene of most isolates was located on the similar genetic structure.
Corresponding author: Gong-Xiang Chen. Mailing Address: 2nd Affiliated Hospital of Zhejiang University, 88 Jiefang Rd, Hangzhou 310009, China; E-mail: email@example.com.
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