17 Jul 2013

Plasmacytoid Dendritic Cells and CD8 T cells from Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection (J Infect Dis., abstract, edited)

[Source: The Journal of Infectious Diseases, full page: (LINK). Abstract, edited.]

Plasmacytoid Dendritic Cells and CD8 T cells from Pregnant Women Show Altered Phenotype and Function Following H1N1/09 Infection

Rebecca L. Vanders 1,2, Peter G. Gibson 1,3, Vanessa E. Murphy 1,2 and Peter A. B. Wark 1,3

Author Affiliations: 1Centre for Asthma and Respiratory Diseases, School of Medicine and Public Health, The University of Newcastle, Newcastle, Australia, 2308 2Hunter Medical Research Institute, Newcastle, Australia, 2305 3Department of Respiratory and Sleep Medicine, John Hunter Hospital, Newcastle, Australia, 2305

Corresponding Author: Dr. Rebecca Vanders, Centre for Asthma and Respiratory Diseases, Level 2, West Wing, Hunter Medical Research Institute, University of Newcastle, University Drive, Callaghan NSW 2308. Phone: 61 2 40420107 Fax: 61 2 40420046, email: Rebecca.Vanders@newcastle.edu.au

 

Abstract

Background.

Pregnant women are a high risk group during influenza pandemics. In this study we determined whether plasmacytoid dendritic cells (pDCs) and CD8 T cells from pregnant women display altered activity following in vitro infection with 2009 pandemic swine influenza (H1N1/09).

Methods.

Peripheral blood mononuclear cells (PBMCs) were isolated from n=26 pregnant and n=28 non-pregnant women. DC subtypes were enumerated from PBMCs. PBMCs were infected with H1N1/09 and CD86, HLA-DR, and Programmed Death Ligand 1/2 (PDL1/2) measured on pDCs. Programmed Death receptor 1 (PD1) was measured on CD8 T cells. IFNα, IL2, TNFα and IFNγ were measured from culture supernatant.

Results.

pDC (i.e.CD303+/CD1cPBMCs) percentages were lower in pregnant compared to non-pregnant women (p<0.05). Following H1N1/09 infection, pDCs from pregnant women showed higher expression of CD86 (p<0.01), HLA-DR (p<0.001) and PDL1 (p<0.001), compared to non-pregnant women. Expression of PD1 on CD8 T cells was also higher during pregnancy (p<0.05). Following H1N1/09 infection, PBMCs from pregnant women displayed reduced IFNα (p<0.01), IL2 (p<0.01) and IFNγ (p<0.01) compared to non-pregnant women, however blocking PDL1 during H1N1/09 infection increased these cytokines from PBMCs of pregnant women (p<0.05).

Conclusion.

Altered maternal cellular antiviral activity is implicated in the increased morbidity during pregnancy following influenza pandemics.

Received January 15, 2013. Revision received March 12, 2013. Accepted March 20, 2013.

© The Author 2013. Published by Oxford University Press on behalf of the Infectious Diseases Society of America. All rights reserved.

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