18 Jun 2013

Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections (mBio, abstract, edited)

[Source: mBio, full page: (LINK). Abstract, edited.]

Identification of a New Cyclovirus in Cerebrospinal Fluid of Patients with Acute Central Nervous System Infections

Le Van Tan a, H. Rogier van Doorn a,b, Ho Dang Trung Nghia a,c,d,  Tran Thi Hong Chau d, Le Thi Phuong Tu a, Michel de Vries e*, Marta Canuti e, Martin Deijs e, Maarten F. Jebbink e, Stephen Baker a,b,  Juliet E. Bryant a,b, Nguyen Thi Tham a, Nguyen Thi Thuy Chinh BKrong a, Maciej F. Boni a,b, Tran Quoc Loi f, Le Thi Phuong f,  Joost T. P. Verhoeven e, Martin Crusat e, Rienk E. Jeeninga e,  Constance Schultsz a,g, Nguyen Van Vinh Chau d, Tran Tinh Hien a,  Lia van der Hoek e, Jeremy Farrar a,b, Menno D. de Jong a,e

Author Affiliations: Oxford University Clinical Research Unit, Wellcome Trust Major Overseas Programme, South East Asia Infectious Diseases Clinical Research Network, Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam (a); Centre for Tropical Medicine, Nuffield Department of Clinical Medicine, University of Oxford, Centre for Clinical Vaccinology and Tropical Medicine, Oxford, United Kingdom (b); Pham Ngoc Thach University of Medicine, Ho Chi Minh City, Vietnam (c); Hospital for Tropical Diseases, Ho Chi Minh City, Vietnam (d); Department of Medical Microbiology, Academic Medical Center, University of Amsterdam, Amsterdam, the Netherlands (e); Dong Thap Provincial Hospital, Dong Thap, Vietnam (f); Department of Global Health-Amsterdam Institute of Global Health and Development, Academic Medical Center, University of Amsterdam, Amsterdam, The Netherlands (h)

Author Notes: * Present address: CBS-KNAW Fungal Biodiversity Center, Utrecht, The Netherlands

Address correspondence to Le Van Tan, tanlv@oucru.org

Invited Editor Amit Kapoor, Columbia University Editor W. Ian Lipkin, Columbia University

 

ABSTRACT

Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology remains unknown in a large proportion of cases. We identified and characterized the full genome of a novel cyclovirus (tentatively named cyclovirus-Vietnam [CyCV-VN]) in cerebrospinal fluid (CSF) specimens of two Vietnamese patients with CNS infections of unknown etiology. CyCV-VN was subsequently detected in 4% of 642 CSF specimens from Vietnamese patients with suspected CNS infections and none of 122 CSFs from patients with noninfectious neurological disorders. Detection rates were similar in patients with CNS infections of unknown etiology and those in whom other pathogens were detected. A similar detection rate in feces from healthy children suggested food-borne or orofecal transmission routes, while high detection rates in feces from pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further research is needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.

IMPORTANCE

Acute central nervous system (CNS) infections cause substantial morbidity and mortality, but the etiology frequently remains unknown, which hampers development of therapeutic or preventive strategies. Hence, identification of novel pathogens is essential and is facilitated by current next-generation sequencing-based methods. Using such technology, we identified and characterized the full genome of a novel cyclovirus in cerebrospinal fluid (CSF) specimens from two Vietnamese patients with CNS infections of unknown etiology, which was subsequently detected in none of 122 CSF specimens from patients with noninfectious neurological disorders but 4% of 642 CSF specimens from Vietnamese patients with suspected or confirmed CNS infections. Similar detection rates in feces from healthy children suggested food-borne or orofecal transmission routes, while frequent detection in feces from Vietnamese pigs and poultry (average, 58%) suggested the existence of animal reservoirs for such transmission. Further studies are needed to address the epidemiology and pathogenicity of this novel, potentially zoonotic virus.

 

FOOTNOTES

Citation Tan LV, van Doorn HR, Nghia HDT, Chau TTH, Tu LTP, de Vries M, Canuti M, Deijs M, Jebbink MF, Baker S, Bryant JE, Tham NT, BKrong NTTC, Boni MF, Loi TQ, Phuong LT, Verhoeven JTP, Crusat M, Jeeninga RE, Schultsz C, Chau NVV, Hien TT, van der Hoek L, Farrar J, de Jong MD. 2013. Identification of a new cyclovirus in cerebrospinal fluid of patients with acute central nervous system infections. mBio 4(3):e00231-13. doi:10.1128/mBio.00231-13.

Received 30 March 2013 - Accepted 9 May 2013 - Published 18 June 2013

Copyright © 2013 Tan et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-ShareAlike 3.0 Unported license, which permits unrestricted noncommercial use, distribution, and reproduction in any medium, provided the original author and source are credited.

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