BOA1

Featured post

#Travel #health #advice on #Zika #virus (@WHO, April 11 2016)

[Source: World Health Organization, full page: ( LINK ).] Travel health advice on Zika virus [       ] 11 April 2016   1. Overview Sin...

25 Feb 2013

Simultaneous Detection of Oseltamivir- and Amantadine-Resistant Influenza by Oligonucleotide Microarray Visualization (PLoS ONE, abstract, edited)

[Source: PLoS ONE, full text: (LINK). Abstract, edited.]

Simultaneous Detection of Oseltamivir- and Amantadine-Resistant Influenza by Oligonucleotide Microarray Visualization

by Yingjie Zhang, Qiqi Liu, Dou Wang, Suhong Chen, Shengqi Wang

 

Presently, the resistance of Influenza A virus isolates causes great difficulty for the prevention and treatment of influenza A virus infection. It is important to establish a drug-resistance detection method for epidemiological study and personalized medicine in the clinical setting. Consequently, a cost-effective oligonucleotide microarray visualization method, which was based on quantum dot-catalyzed silver deposition, was developed and evaluated for the simultaneous detection of neuraminidase H275Y and E119V; matrix protein 2 V27A and S31N mutations of influenza A (H3N2), seasonal influenza A (H1N1), and 2009 influenza A (H1N1). Then, 307 clinical throat swab specimens were detected and the drug-resistance results showed that 100% (17/17) of influenza A (H3N2) and 100% (259/259) of 2009 influenza A (H1N1) samples were resistant to amantadine and susceptible to oseltamivir; and 100% (5/5) of seasonal influenza A (H1N1) samples were resistant to both amantadine and oseltamivir.

-

------