18 Feb 2013

Molecular Epidemiology and Complete Genome Characterization of H1N1pdm Virus from India (PLoS ONE, abstract, edited)

[Source: PLoS ONE, full text: (LINK). Abstract, edited.]

Research Article

Molecular Epidemiology and Complete Genome Characterization of H1N1pdm Virus from India

Shashi Sharma, Gaurav Joshi, Paban K. Dash, Maria Thomas, Thimmasandra N. Athmaram, Jyoti S. Kumar, Anita Desai, Ravi Vasanthapuram, Ishan K. Patro, Putcha V. L. Rao, Manmohan Parida

Affiliation: [Full list on source page.]

 

Abstract

Background

Influenza A virus is one of world’s major uncontrolled pathogen, causing seasonal epidemic as well as global pandemic. This was evidenced by recent emergence and continued prevalent 2009 swine origin pandemic H1N1 Influenza A virus, provoking first true pandemic in the past 40 years. In the course of its evolution, the virus acquired many mutations and multiple unidentified molecular determinants are likely responsible for the ability of the 2009 H1N1 virus to cause increased disease severity in humans. Availability of limited data on complete genome hampers the continuous monitoring of this type of events. Outbreaks with considerable morbidity and mortality have been reported from all parts of the country.

Methods/Results

Considering a large number of clinical cases of infection complete genome based sequence characterization of Indian H1N1pdm virus and their phylogenetic analysis with respect to circulating global viruses was undertaken, to reveal the phylodynamic pattern of H1N1pdm virus in India from 2009–2011. The Clade VII was observed as a major circulating clade in phylogenetic analysis. Selection pressure analysis revealed 18 positively selected sites in major surface proteins of H1N1pdm virus.

Conclusions

This study clearly revealed that clade VII has been identified as recent circulating clade in India as well globally. Few clade VII specific well identified markers undergone positive selection during virus evolution. Continuous monitoring of the H1N1pdm virus is warranted to track of the virus evolution and further transmission. This study will serve as a baseline data for future surveillance and also for development of suitable therapeutics.

 

Citation: Sharma S, Joshi G, Dash PK, Thomas M, Athmaram TN, et al. (2013) Molecular Epidemiology and Complete Genome Characterization of H1N1pdm Virus from India. PLoS ONE 8(2): e56364. doi:10.1371/journal.pone.0056364

Editor: Jean-Pierre Vartanian, Institut Pasteur, France

Received: October 12, 2012; Accepted: January 8, 2013; Published: February 15, 2013

Copyright: © 2013 Sharma et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

Funding: This work was funded by Defence Research and Development Establishment (DRDE), Gwalior an Institute under ministry of defence, Government of India. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

Competing interests: The authors have declared that no competing interests exist.

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